In order to utilize the agricultural waste products, two strains of mold producing powerful cellulolytic enzyme were screened from various soils, composts, rotten wood and others. The optimum condition of cellulase production was studied. The results obtained were summarized as follows.
1. Two strains of mold which showed remarkably high cellulolytic activity were identified as Aspergillus niger-SM 6 and Trichoderma viride-SM 10.
2. The highest cellulase production was obtained at pH 5.0-6.0 in 5 days.
3. Cellulase production in strain Aspergillus niger-SM 6 increased with the addition of C.M.C., (NH₄)₂SO₄, C.S.L., orange peel powder and rice hull. The rice hull, treated with 3N NaOH at 120℃ for 15 min. and neutralized with various acids, was used. Up to 50% of wheat bran could be substituted by the treated rice hull without any-decrease of cellulase activity.
4. In the strain of Trichoderma viride-SM 10, cellulase production increased with the addition of C.M.C., NH₄NO₃, Vitamin-free casamino acid and orange peel powder, while the other carbon, nitrogen, phosphate sources, natural nutrients and organic substances gave no remarkable effect.
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